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Updated: May 29, 2026

A FACS-based Protocol to Isolate RNA from the Secondary Cells of Drosophila Male Accessory Glands
Published on: September 5, 2019
LUMP is a putative double-stranded RNA binding protein required for male fertility in Drosophila melanogaster
Charcacia Sanders1, Dean P Smith
1Department of Pharmacology and Neuroscience, University of Texas Southwestern Medical Center, Dallas, Texas, United States of America.
Abstract:
In animals, male fertility requires the successful development of motile sperm. During Drosophila melanogaster spermatogenesis, 64 interconnected spermatids descended from a single germline stem cell are resolved into motile sperm in a process termed individualization. Here we identify a putative double-stranded RNA binding protein LUMP that is required for male fertility. lump(1) mutants are male-sterile and lack motile sperm due to defects in sperm individualization. We show that one dsRNA binding domains (dsRBD) is essential for LUMP function in male fertility. These findings reveal LUMP is a novel factor required for late stages of male germline differentiation.
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