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Immunogold Electron Microscopy

Immunogold Electron Microscopy

Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.

Preparation of Samples for Electron Microscopy

Preparation of Samples for Electron Microscopy

To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...

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Related Experiment Video

Updated: May 29, 2026

Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration

Visualizing Synaptic Degeneration in Adult Drosophila in Association with Neurodegeneration

Published on: May 13, 2020

Degenerating processes identified by electron microscopic immunocytochemical methods.

Teresa A Milner¹, Elizabeth M Waters, Danielle C Robinson

¹Division of Neurobiology, Department of Neurology and Neuroscience, Weill Cornell Medical College, New York, NY, USA. tmilner@med.cornell.edu

Methods in Molecular Biology (Clifton, N.J.)

|September 14, 2011

Summary

This summary is machine-generated.

Electron microscopic immunolabeling offers early detection of neural degeneration. This technique identifies apoptotic and disease markers in neural and glial cells, improving analysis of degenerative mechanisms.

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Area of Science:

Neuroscience
Cell Biology
Immunohistochemistry

Background:

Traditional ultrastructural analysis of neural degeneration reveals changes only after significant cellular atrophy.
Early degenerative processes in neural tissue were difficult to identify at the subcellular level.

Purpose of the Study:

To enhance the identification and analysis of early-stage degenerative processes in neural tissue.
To improve the characterization of apoptosis and other neurodegenerative mechanisms.

Main Methods:

Application of electron microscopic immunolabeling techniques.
Utilizing antibodies against degenerative markers like cytoplasmic cytochrome c, beta-amyloid, and Iba1.
Employing dual immunolabeling for detailed analysis.

Main Results:

Degenerating neural and glial processes are identified via specific antibody labeling.
Subcellular distribution of markers and relationships to surrounding elements are characterized.
Dual immunolabeling provides additional data on process phenotype and inputs.

Conclusions:

Electron microscopic immunolabeling significantly advances the study of early neural degeneration.
This method allows for detailed examination of apoptotic and pathological processes.
The technique refines understanding of degenerative mechanisms in neural and glial cells.