A genetic in vivo system to detect asymmetrically distributed RNA
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Updated: May 29, 2026

A Rapid High-throughput Method for Mapping Ribonucleoproteins (RNPs) on Human pre-mRNA
Published on: December 2, 2009
N JayaNandanan1, Elizabeth R Gavis, Veit Riechmann
1Institute of Genetics, University of Cologne, Zülpicher Strasse 47, Cologne 50674, Germany.
Abstract:
Many RNAs show polarized or otherwise non-random subcellular distributions. To create a method for genome-wide genetic screens for RNAs with asymmetric subcellular distributions, we have combined methods for gene tagging and live imaging of messenger RNA (mRNA). A pilot screen in a highly polarized, differentiated cell in the Drosophila larva, the branched terminal cell of the tracheal system, demonstrates the feasibility of the method for identifying new asymmetrically localized mRNAs in vivo.
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