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Updated: May 29, 2026

Multiplex PCR and Reverse Line Blot Hybridization Assay (mPCR/RLB)
Published on: August 6, 2011
The real-time PCR for sensitive protein detection by target-induced intermolecular hybridization
Cuiping Ma1, Lijie Cao, Chao Shi
1State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, PR China.
Abstract:
An approach was developed to sensitively and specially detect protein in buffer and serum based on target-induced intermolecular hybridization and the significant difference of duplex stability in intramolecular versus intermolecular.
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