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Genetic Screens

Genetic Screens

Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
Forward genetic screens
Forward or “classical” genetic screens involve creating random mutations in an organism’s DNA using radiation, mutagens, or insertion of additional bases, which result in visible changes...

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: May 29, 2026

Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe

Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe

Published on: September 13, 2022

Genetic suppressor screens in haploids.

Xiaoying Bai¹, Zhongan Yang, Hong Jiang

¹Howard Hughes Medical Institute, Boston, Massachusetts, USA.

Methods in Cell Biology

|September 20, 2011

Summary

Zebrafish modifier screens identified a new transcriptional mechanism controlling red blood cell formation (erythropoiesis). This study demonstrates the power of modifier screens and haploid genetics in zebrafish for biological discovery.

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Production of Haploid Zebrafish Embryos by In Vitro Fertilization

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Published on: July 14, 2014

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Last Updated: May 29, 2026

Genetic Screen for Identification of Multicopy Suppressors in Schizosaccharomyces pombe

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A Deep-sequencing-assisted, Spontaneous Suppressor Screen in the Fission Yeast Schizosaccharomyces pombe

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Production of Haploid Zebrafish Embryos by In Vitro Fertilization

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Published on: July 14, 2014

Area of Science:

* Vertebrate genetics
* Developmental biology
* Human disease modeling

Background:

* Zebrafish are a key vertebrate model for studying biological processes and diseases.
* Forward genetic screens yield mutants with disease-like phenotypes, but mechanisms often remain unclear.
* Modifier screens can identify genes that influence these phenotypes, aiding mechanistic studies.

Purpose of the Study:

* To describe the first genetic suppressor screen in zebrafish.
* To identify novel genetic mechanisms regulating erythropoiesis (red blood cell development).
* To demonstrate the utility of modifier screens combined with haploid genetics in zebrafish.

Main Methods:

* Performed a genetic suppressor screen in zebrafish.
* Utilized haploid genetics to enhance the screen's efficiency.
* Analyzed mutations that modify phenotypes of existing zebrafish mutants.

Main Results:

* Identified a novel transcriptional mechanism regulating erythropoiesis.
* Demonstrated the feasibility and effectiveness of modifier screens in zebrafish.
* Validated the combined approach of modifier screens and haploid genetics.

Conclusions:

* Modifier screens are a powerful strategy for elucidating genetic mechanisms in zebrafish.
* This approach significantly enhances zebrafish's utility for biological discovery.
* The identified transcriptional mechanism provides new insights into erythropoiesis regulation.