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Related Experiment Video

Updated: May 29, 2026

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
07:16

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Published on: August 18, 2022

DNA methylation profiling in zebrafish.

Shan-Fu Wu1, Haiying Zhang, Saher Sue Hammoud

  • 1Howard Hughes Medical Institute, Chevy Chase, Maryland, USA.

Methods in Cell Biology
|September 20, 2011
PubMed
Summary
This summary is machine-generated.

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Zebrafish DNA methylation is a dynamic epigenetic process crucial for gene regulation. This study explores methods like MeDIP and shotgun bisulphite sequencing to quantify DNA methylation genome-wide in zebrafish.

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Genomics

Background:

  • DNA methylation on cytosine is a key epigenetic modification regulating gene expression in vertebrates.
  • This process involves gene silencing via transcription factor interference and repressive chromatin recruitment.
  • Zebrafish are an excellent model organism due to conserved DNA methylation machinery.

Purpose of the Study:

  • To discuss primary methods for quantifying genome-wide DNA methylation levels in zebrafish.
  • To introduce analytical approaches for identifying methylation patterns and differentially methylated regions.

Main Methods:

  • Methylated DNA immunoprecipitation (MeDIP) for regional methylation analysis.
  • Shotgun bisulphite sequencing for base-pair resolution DNA methylation quantification.

More Related Videos

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
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Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

Related Experiment Videos

Last Updated: May 29, 2026

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos
07:16

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Published on: August 18, 2022

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System
10:17

Profiling DNA Replication Timing Using Zebrafish as an In Vivo Model System

Published on: April 30, 2018

Main Results:

  • The study outlines established and emerging techniques for DNA methylation analysis in zebrafish.
  • It also details analytical tools for identifying hypo- and hyper-methylated regions.

Conclusions:

  • Zebrafish provide a valuable model for studying DNA methylation's role in gene regulation.
  • Accurate quantification and analysis of DNA methylation are essential for understanding epigenetic control.