Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

TGF - β Signaling Pathway

TGF - β Signaling Pathway

The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...

GPCRs Regulate Adenylyl Cylase Activity

GPCRs Regulate Adenylyl Cylase Activity

Some GPCRs transmit signals through adenylyl cyclase (AC), a transmembrane enzyme. AC helps synthesize second messenger cyclic adenosine monophosphate (cAMP). AC catalyzes cyclization reaction and converts ATP to cAMP by releasing a pyrophosphate. The pyrophosphate is further hydrolyzed to phosphate by the enzyme pyrophosphatase, which drives cAMP synthesis to completion. However, cAMP is rapidly degraded to 5′ AMP by the enzymes phosphodiesterase (PDE), preventing overstimulation of cells.
Two...

Mitogens and the Cell Cycle

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...

Cells Coordinate Growth and Proliferation

Cells Coordinate Growth and Proliferation

Cell size is a significant factor impacting cellular design, function, and fitness. There exists some internal coordination by which cells double their masses before division, thus, achieving homeostasis. Coordination between cell growth and proliferation depends on the checkpoints in between cell cycle phases. Loss of coordination or failure in the checkpoint mechanism can drive the cell to uncontrolled growth and loss of cellular function. Like dividing cells that coordinate cellular growth,...

Activation and Inactivation of G Proteins

Activation and Inactivation of G Proteins

Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high affinity and are together...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Impact of metal exchange on the electronic structure and optical properties of isostructural octa-coordinated Mo<sup>IV</sup>/Cd<sup>II</sup> and W<sup>IV</sup>/Cd<sup>II</sup> polynuclear cyanide polymers.

Dalton transactions (Cambridge, England : 2003)·2024

Same author

Obesity drives adipose-derived stem cells into a senescent and dysfunctional phenotype associated with P38MAPK/NF-KB axis.

Immunity & ageing : I & A·2023

Same author

Rutile nano-bio-interactions mediate dissimilar intracellular destiny in human skin cells.

Nanoscale advances·2022

Same author

Comparison of the Effect of Agitation on Whitening and Tooth Sensitivity of In-Office Bleaching: A Randomized Clinical Trial.

Operative dentistry·2021

Same author

Sonic Activation of a Desensitizing Gel Prior to In-Office Bleaching.

Operative dentistry·2021

Same author

Comparison of the Effect of Agitation on Whitening and Tooth Sensitivity of In-Office Bleaching: A Randomized Clinical Trial.

Operative dentistry·2021

Search research articles

Related Experiment Video

Updated: May 29, 2026

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

GD1a modulates GM-CSF-induced cell proliferation.

A X S Santos¹, J E Maia, P M Crespo

¹Laboratório de Bioquímica e Biologia Celular de Lipídios, Depto Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil. alinexss@gmail.com

|September 21, 2011

Summary

Ganglioside GD1a enhances granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced proliferation in myeloid cells. This suggests GD1a

More Related Videos

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (T_HGM) Cells

Published on: September 10, 2018

Related Experiment Videos

Last Updated: May 29, 2026

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (THGM) Cells

In Vitro Differentiation of Mouse Granulocyte-macrophage-colony-stimulating Factor (GM-CSF)-producing T Helper (T_HGM) Cells

Published on: September 10, 2018

Area of Science:

Hematology
Cell Biology
Immunology

Background:

Gangliosides are implicated in cell proliferation and differentiation, particularly in hematopoietic cells.
Previous studies showed gangliosides support myeloid precursor cell proliferation.
Stromal cells shed gangliosides, which are incorporated into myeloid cell membranes.

Purpose of the Study:

To investigate the effect of gangliosides on granulocyte-macrophage colony-stimulating factor (GM-CSF)-induced proliferation.
To explore the role of ganglioside GD1a in myeloid cell proliferation.

Main Methods:

Utilized the monocytic FDC-P1 cell line, dependent on GM-CSF.
Cultured cells with GM-CSF and exogenous gangliosides (GM3, GD1a, GM1) or with a ceramide-synthase inhibitor (D-PDMP).
Assessed proliferation, GM-CSF receptor alpha (GMRα) expression, C/EBPα levels, and GD1a/GMR co-localization via immunocytochemistry.

Main Results:

Exogenous GD1a significantly enhanced GM-CSF-induced proliferation of FDC-P1 cells.
Observed increased expression of GMRα and the transcription factor C/EBPα with GD1a addition.
Inhibition of endogenous ganglioside synthesis reduced proliferation, which was restored by exogenous GD1a.
Demonstrated co-localization of GD1a and GMR.

Conclusions:

Ganglioside GD1a modulates GM-CSF-mediated proliferative responses.
GD1a's role in hematopoiesis is significant.
Potential implications in immunology, Alzheimer disease, alveolar proteinosis, and other GM-CSF-related processes.