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Updated: May 29, 2026

Photoacoustic Cystography
Published on: June 11, 2013
Nonionizing photoacoustic cystography in vivo
Chulhong Kim1, Mansik Jeon, Lihong V Wang
1BioOptics and Acoustics Laboratory, Department of Biomedical Engineering, University at Buffalo, The State University of New York, Buffalo, New York 14260, USA. chulhong@buffalo.edu
Abstract:
We demonstrate the feasibility of a novel and nonionizing process for bladder imaging in vivo, called photoacoustic cystography (PAC). Using a photoacoustic imaging system, we have successfully imaged a rat bladder filled with clinically used Methylene Blue (MB) dye. An image contrast of ~8 was achieved. Further, spectroscopic PAC confirmed the accumulation of MB in the bladder. Using a laser pulse energy of less than 1 mJ/cm² (1/20 of the ANSI safety limit), a deeply (1.2 cm) positioned bladder in biological tissues was clearly visible in the PA image. Our results suggest that PAC can potentially provide a nonionizing, relatively cheap, and portable tool for bladder mapping. Among our clinical interests, nonionizing PAC with an injection of MB can potentially monitor vesicoureteral reflux in children.
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