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Updated: May 29, 2026

Structural Studies of Macromolecules in Solution using Small Angle X-Ray Scattering
Published on: November 5, 2018
Measuring protein structure and stability of protein-nanoparticle systems with synchrotron radiation circular
Stefania Laera1, Giacomo Ceccone, Francois Rossi
1European Commission, Joint Research Centre, Institute for Health and Consumer Protection I-21027, Ispra (VA), Italy.
Abstract:
We measure the structural and stability changes of proteins at nanomolar concentration upon interaction with nanoparticles. Using synchrotron radiation circular dichroism (SRCD), we measure a decrease of 6 °C in the thermal unfolding of human serum albumin upon interaction with silver nanoparticles while this does not happen with gold. The use of SRCD allows measuring critical parameters on protein-nanoparticle interactions, and it will provide experimental data on the relative stability of key biological proteins for nanotoxicology.

