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Updated: Aug 28, 2026

AQRNA-seq for Quantifying Small RNAs
Published on: February 2, 2024
Critical Evaluation of Key Methods for RNA Quantification
Valentina Paracchini1, Mauro Petrillo2, Luca Fornara3
1European Commission, Joint Research Centre (JRC), 2440 Geel, Belgium.
Abstract:
The approval of safe and effective lipid nanoparticles-encapsulated mRNA (LNP-mRNA) vaccines during the SARS-CoV-2 pandemic is catalysing the development of the next generation of mRNA therapeutics. Accurate characterisation methods are crucial for assessing the quality and efficacy of these complex formulations. Several analytical techniques exist for the quantification of the mRNA drug substance, including UV spectroscopy, capillary electrophoresis, high-performance liquid chromatography (HPLC), and digital PCR (dPCR). Here, we compare these methods, highlight their limitations, and discuss critical considerations for experimental design to improve the agreement between different analytical methods.
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