Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 29, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

Delayed osteoblastic differentiation and bone development in Cx43 knockout mice.

Lucas Martins Chaible1, Daniel Soares Sanches, Bruno Cogliati

  • 1Department of Pathology, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Sao Paulo, Brazil. chaible@usp.br

Toxicologic Pathology
|September 22, 2011
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sexual dimorphism in the association of circulating TAM receptors-ligands with MASLD-related fibrosis.

JHEP reports : innovation in hepatology·2026
Same author

Canine cutaneous and subcutaneous soft tissue sarcoma in dogs: a consensus report from the Brazilian association of veterinary oncology.

Frontiers in veterinary science·2026
Same author

Comprehensive kinase inhibitor screening reveals potential therapeutic targets in canine melanoma: A Comparative Precision Oncology study.

Research in veterinary science·2026
Same author

Residual bile flow shapes PPARγ-mediated antifibrotic responses in experimental segmental cholestasis.

Scientific reports·2026
Same author

Risk factors associated with solid oral tumors in dogs: a case-control study in São Paulo, Brazil.

Frontiers in veterinary science·2026
Same author

An immunocompetent murine model of virus-elicited liver fibrosis and hepatocellular carcinoma.

Journal of hepatology·2026

The GJA1 gene (Connexin43) is crucial for fetal bone development. Its deficiency causes delays in osteoblast differentiation and bone matrix formation in mice, with potential compensatory roles for other connexins.

Area of Science:

  • Developmental Biology
  • Genetics
  • Biochemistry

Background:

  • The GJA1 gene encodes Connexin43 (Cx43), a key gap junction protein vital for intercellular communication.
  • Cx43 is implicated in embryonic bone development, but its precise role in osteogenesis requires further elucidation.

Purpose of the Study:

  • To investigate the in vivo effects of GJA1 gene deficiency on fetal bone development.
  • To identify histological and molecular changes associated with partial or complete GJA1 deletion in mouse fetuses.

Main Methods:

  • Utilized heterozygous (GJA1(+/-)) and homozygous (GJA1(-/-)) knockout mice, alongside wild-type littermates.
  • Conducted histological, histochemical, and real-time PCR analyses to assess bone development markers.
  • Monitored fetal growth and development from 12.5 to 19.5 days post coitum.

More Related Videos

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
08:42

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement
05:25

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

Published on: July 21, 2023

Related Experiment Videos

Last Updated: May 29, 2026

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis
09:20

Laser Capture Microdissection of Mouse Embryonic Cartilage and Bone for Gene Expression Analysis

Published on: December 18, 2019

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model
08:42

Skeletal Phenotype Analysis of a Conditional Stat3 Deletion Mouse Model

Published on: July 3, 2020

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement
05:25

Using Inducible Osteoblastic Lineage-Specific Stat3 Knockout Mice to Study Alveolar Bone Remodeling During Orthodontic Tooth Movement

Published on: July 21, 2023

Main Results:

  • GJA1-deficient fetuses (both heterozygous and homozygous) displayed delayed osteoblast differentiation and reduced bone matrix deposition.
  • Homozygous GJA1 knockout fetuses showed persistent developmental delays, while heterozygous fetuses normalized post-17.5 dpc.
  • Expression of key bone development genes (osteocalcin, osteopontin, alkaline phosphatase, RUNX2) and other connexin genes (GJC1, GJA3) was significantly delayed in GJA1-deficient models.

Conclusions:

  • GJA1 plays a critical role in regulating the timing and progression of fetal bone development.
  • GJC1 (Cx45) may contribute to restoring intercellular communication in the absence of GJA1 (Cx43).
  • This study enhances understanding of GJA1's function in osteogenesis and highlights potential compensatory mechanisms.