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Related Experiment Videos

Decrease in rat submandibular acinar cell volume during ACh stimulation.

T Nakahari1, M Murakami, H Yoshida

  • 1Department of Physiology, Osaka Medical College, Takatsuki, Japan.

The American Journal of Physiology
|June 1, 1990
PubMed
Summary

Salivary gland cells shrink during acetylcholine-induced secretion, with volume decreasing significantly. Cell volume recovery indicates fluid influx, suggesting a transcellular route for salivary secretion.

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Area of Science:

  • Physiology
  • Cell Biology
  • Glandular Secretion

Background:

  • Salivary glands produce saliva, essential for digestion and oral health.
  • Understanding the cellular mechanisms of salivary secretion is crucial for treating related disorders.

Purpose of the Study:

  • To investigate changes in acinar cell volume during stimulated salivary secretion.
  • To determine the fluid flux across acinar cells and identify secretion pathways.

Main Methods:

  • Perfused rat submandibular gland preparation.
  • Measurement of acinar cell volume using impedance and morphometric methods.
  • Stimulation with acetylcholine (ACh) at 1 microM.

Main Results:

  • Acinar cell volume decreased significantly during ACh-induced secretion (approx. 70-76% of resting volume).

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  • Cell volume recovered to above resting levels upon ACh removal, indicating fluid influx.
  • Gland weight decreased, correlating with fluid secretion.

    • Conclusions:

    • Acinar cell shrinkage is a key event during stimulated salivary secretion.
    • Fluid influx across the basolateral membrane occurs during secretion.
    • Evidence supports a transcellular route for fluid secretion in salivary glands.