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Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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Lensless Fluorescent Microscopy on a Chip
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Published on: August 17, 2011

Fluorescence microscopy imaging with a Fresnel zone plate array based optofluidic microscope.

Shuo Pang1, Chao Han, Lap Man Lee

  • 1Department of Electrical Engineering, California Institute of Technology, Pasadena, CA 91125, USA. spang@caltech.edu

Lab on a Chip
|September 22, 2011
PubMed
Summary
This summary is machine-generated.

We developed a fluorescence optofluidic microscope (FOFM) for imaging fluid samples. This on-chip system uses Fresnel zone plates and a CMOS sensor to achieve 1.0 μm resolution for biological imaging.

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Area of Science:

  • Optics and Photonics
  • Biomedical Engineering
  • Microscopy

Background:

  • Traditional fluorescence microscopy is limited in high-throughput analysis of fluid samples.
  • Developing miniaturized, integrated microscopy systems is crucial for point-of-care diagnostics and cell analysis.

Purpose of the Study:

  • To implement and demonstrate an on-chip fluorescence optofluidic microscope (FOFM) for imaging samples in fluid media.
  • To achieve high-resolution fluorescence imaging using a microfluidic platform.

Main Methods:

  • An array of Fresnel zone plates (FZP) was used to generate focused light spots within a microfluidic channel.
  • Fluorescence emissions from flowing samples were collected by a filter-coated CMOS sensor.
  • Image reconstruction was performed based on the collected fluorescence data.

Main Results:

  • The system achieved a resolution of 1.0 μm, with an experimentally measured focused spot size of 0.65 μm.
  • Demonstrated imaging of cell nuclei stained with Acridine Orange.
  • Showcased imaging of cytoplasm labeled with Qtracker®.

Conclusions:

  • The developed FOFM system is capable of high-resolution fluorescence microscopy of biological samples in microfluidic channels.
  • This on-chip microscopy approach offers potential for advanced biological sample analysis and diagnostics.