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Related Concept Videos

Restriction Enzymes01:11

Restriction Enzymes

Restriction enzymes are bacterial enzymes used to cut DNA in a sequence-specific manner. To cleave DNA, they bind to specific palindromic sequences called restriction sites. Such palindromic DNA sequences or inverted repeats are commonly found in regions of functional significance, such as the origin of replication, gene operator sites, and regions containing transcription termination signals.
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Mismatch Repair01:20

Mismatch Repair

Organisms are capable of detecting and fixing nucleotide mismatches that occur during DNA replication. This sophisticated process requires identifying the new strand and replacing the erroneous bases with correct nucleotides. Mismatch repair is coordinated by many proteins in both prokaryotes and eukaryotes.
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RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...
DNA Helicases00:55

DNA Helicases

DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

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An N-terminal helix of Lsm11 stabilizes CPSF73 in U7 snRNP for histone pre-mRNA 3'-end processing.

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Proteins that recognize unique features of U7 snRNA and may substitute for Gemin5 in the assembly of U7-specific Sm ring.

RNA (New York, N.Y.)·2025
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Anticancer benzoxaboroles block pre-mRNA processing by directly inhibiting CPSF3.

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In vitro methylation of the U7 snRNP subunits Lsm11 and SmE by the PRMT5/MEP50/pICln methylosome.

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<i>In vitro</i> methylation of the U7 snRNP subunits Lsm11 and SmE by the PRMT5/MEP50/pICln methylosome.

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Related Experiment Video

Updated: May 29, 2026

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage
09:53

Single-Molecule Dwell-Time Analysis of Restriction Endonuclease-Mediated DNA Cleavage

Published on: February 7, 2021

The hunt for the 3' endonuclease.

Zbigniew Dominski1

  • 1Department of Biochemistry and Biophysics and Program in Molecular Biology and Biotechnology, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA. dominski@med.unc.edu

Wiley Interdisciplinary Reviews. RNA
|September 22, 2011
PubMed
Summary

Cleavage and polyadenylation factor 73 (CPSF-73) is identified as the endonuclease in both standard mRNA 3' end processing and histone pre-mRNA cleavage. This suggests a shared evolutionary origin for these crucial RNA processing pathways.

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Last Updated: May 29, 2026

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Area of Science:

  • Molecular Biology
  • RNA Processing
  • Biochemistry

Background:

  • Pre-mRNA 3' end processing typically involves cleavage and polyadenylation, a two-step reaction.
  • Replication-dependent histone transcripts in metazoans use a distinct U7 snRNP-dependent cleavage mechanism without polyadenylation.
  • The endonuclease responsible for these cleavage events remained unidentified until recently.

Purpose of the Study:

  • To identify the endonuclease involved in pre-mRNA 3' end processing.
  • To investigate the role of CPSF-73 in both polyadenylated mRNA and histone pre-mRNA processing.

Main Methods:

  • Bioinformatic analysis to identify potential endonucleases.
  • In silico structural analysis of candidate proteins.
  • Experimental validation of endonuclease activity in vitro and in vivo.

Main Results:

  • Bioinformatics and structural analysis implicated CPSF-73, a metallo-β-lactamase family member, as the endonuclease.
  • Experimental evidence supports CPSF-73's role in polyadenylated mRNA processing, though some controversy exists regarding CPSF-30 activity.
  • CPSF-73 was also identified as the endonuclease in U7-dependent histone pre-mRNA processing.

Conclusions:

  • CPSF-73 functions as the endonuclease in both canonical mRNA 3' end cleavage and histone pre-mRNA cleavage.
  • These two distinct RNA processing pathways likely evolved from a common ancestral mechanism centered on CPSF-73.