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Updated: May 29, 2026
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Fabrication of Extracellular Matrix-derived Foams and Microcarriers as Tissue-specific Cell Culture and Delivery Platforms
Published on: April 11, 2017
Cultured cell-derived extracellular matrix scaffolds for tissue engineering.
Hongxu Lu1, Takashi Hoshiba, Naoki Kawazoe
1Tissue Regeneration Materials Unit, International Center for Materials Nanoarchitectonics, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.
Biomaterials
|September 23, 2011
Summary
Cell-derived extracellular matrix (ECM) scaffolds promote tissue regeneration. These scaffolds, made from mesenchymal stem cells, chondrocytes, or fibroblasts, effectively support cell growth and differentiation for cartilage and skin tissue engineering.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Regenerative Medicine
Background:
- Cell-derived extracellular matrix (ECM) scaffolds are promising for tissue regeneration.
- Mesenchymal stem cells (MSCs), chondrocytes, and fibroblasts are key cell types for tissue engineering.
- Poly(lactic-co-glycolic acid) (PLGA) templates offer a method for scaffold fabrication.
Purpose of the Study:
- To investigate the potential of cell-derived ECM scaffolds for cartilage and skin tissue engineering.
- To evaluate the efficacy of MSC-, chondrocyte-, and fibroblast-derived ECM scaffolds in supporting cell growth and differentiation.
- To compare the performance of ECM scaffolds with conventional tissue engineering methods.
Main Methods:
- ECM scaffolds were prepared using MSCs, chondrocytes, and fibroblasts cultured on PLGA templates.
- MSCs were cultured on MSC- and chondrocyte-derived ECM scaffolds.
- Fibroblasts were cultured on fibroblast-derived ECM scaffolds.
Main Results:
- ECM scaffolds supported cell adhesion, proliferation, and ECM production.
- MSC- and chondrocyte-derived ECM scaffolds enhanced MSC chondrogenesis compared to pellet cultures.
- Cartilage-like tissues were regenerated when MSCs were cultured on ECM scaffolds.
- Fibroblasts proliferated and formed uniform multilayered tissue on fibroblast-derived ECM scaffolds.
Conclusions:
- Cell-derived ECM scaffolds facilitate tissue regeneration.
- These scaffolds are a valuable tool for cartilage and skin tissue engineering applications.
- The use of specific cell-derived ECM can promote desired tissue formation.
