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Related Experiment Video

Updated: May 29, 2026

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)
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Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

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Detecting DNaseI-hypersensitivity sites with MLPA.

Thomas Ohnesorg1, Stefanie Eggers, Stefan J White

  • 1Molecular Development Laboratory, Murdoch Childrens Research Institute, Royal Children's Hospital, Melbourne, VIC, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|September 23, 2011
PubMed
Summary

Researchers developed a new, cost-effective method to analyze DNaseI-hypersensitive sites in chromatin. This technique enables high-throughput analysis of up to 50 genomic regions in a single PCR reaction.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Epigenetics

Background:

  • DNaseI-hypersensitive sites indicate regulatory genomic regions.
  • Existing methods for analyzing these sites are often laborious, low-throughput, or expensive.

Purpose of the Study:

  • To develop a novel, efficient, and cost-effective method for analyzing DNaseI-hypersensitive sites.
  • To enable high-throughput analysis of multiple genomic regions simultaneously.

Main Methods:

  • A modified version of multiplex ligation-dependent probe amplification (MLPA) was developed.
  • The approach utilizes a single, PCR-based reaction.
  • It allows analysis of up to 50 defined genomic regions.

Main Results:

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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
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Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

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Last Updated: May 29, 2026

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)
11:35

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay (EMSA) and DNA-affinity Precipitation Assay (DAPA)

Published on: August 21, 2016

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids
06:53

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

  • The developed method provides a new approach to analyzing DNaseI-hypersensitive sites.
  • It offers a more efficient and potentially less expensive alternative to existing techniques.
  • The method facilitates high-throughput screening of genomic regulatory regions.

    • Conclusions:

    • The modified MLPA technique is a valuable tool for studying DNaseI-hypersensitive sites.
    • This approach enhances the efficiency and throughput of regulatory genomic region analysis.
    • It offers a practical solution for researchers investigating chromatin accessibility and gene regulation.