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Updated: Jan 25, 2026

Tissue Collection of Bats for -Omics Analyses and Primary Cell Culture
Published on: October 23, 2019
[Modeling of kidney collecting ducts function in the cell culture conditions]
Abstract:
Continuous cell lines originating from the kidney collecting duct represent a powerful tool for the modeling of water and ions reabsorbtion processes. Present review considers the basic methodical approaches being utilized to study vasopressin-induced water transport mechanisms in the cell culture conditions--microscopical methods, electrophysiological measurements, various ways of evaluation of water flow across the cell monolayer, transfections of native and mutant proteins, GFP-technology. The results of the highest significance for the understanding of collecting ducts function which were obtained with usage of these methods are analyzed in the review.
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