Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
Spindle Assembly02:50

Spindle Assembly

Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
In most cells, centrosomes are the primary microtubule nucleation centers. In the centrosome-mediated pathway, the G2-prophase transition triggers centrosome maturation and increased microtubule nucleation. Progressive nucleation results in a microtubule array...
Separation of Sister Chromatids02:17

Separation of Sister Chromatids

At the transition from prophase to metaphase, there is a reduction in cohesion along the chromosomal arms, resulting in the resolution of sister chromatids. However, residual cohesin connections remain to hold the sister chromatids together until the transition from metaphase to anaphase. The residual connection prevents any premature separation of sister chromatids, blocking the risks of aneuploidy within the daughter cells.
At the onset of anaphase, separase, a proteolytic enzyme, is...
Disassembly of Intermediate Filaments01:35

Disassembly of Intermediate Filaments

Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
Cohesins02:20

Cohesins

Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of homologous...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Progerin-induced nuclear envelope remodeling is shaped by cell division and NUP153.

Journal of cell science·2026
Same author

RNA splicing is required for timely completion of abscission and is modulated by the abscission checkpoint.

bioRxiv : the preprint server for biology·2025
Same author

The Calpain-7 protease functions together with the ESCRT-III protein IST1 within the midbody to regulate the timing and completion of abscission.

eLife·2023
Same author

The dynamic nuclear envelope: resilience in health and dysfunction in disease.

Current opinion in cell biology·2023
Same author

Nuclear pore complexes concentrate on Actin/LINC/Lamin nuclear lines in response to mechanical stress in a SUN1 dependent manner.

Heliyon·2023
Same author

Comprehensive analysis of the human ESCRT-III-MIT domain interactome reveals new cofactors for cytokinetic abscission.

eLife·2022
Same journal

The nucleus as a mechanobiological hub in muscle aging.

Nucleus (Austin, Tex.)·2026
Same journal

Intrinsically disordered regions facilitate Mlp1-Nab2 recognition in mRNA quality control.

Nucleus (Austin, Tex.)·2026
Same journal

High-resolution nuclear cell biology by cryo-electron tomography.

Nucleus (Austin, Tex.)·2026
Same journal

Nuclei on the move: LINC complex-dependent and -independent mechanisms of nuclear migration in development.

Nucleus (Austin, Tex.)·2026
Same journal

Integration of nuclear mechanosensing with integrin-extracellular matrix adhesions.

Nucleus (Austin, Tex.)·2026
Same journal

Antagonistic contributions of A-type and B-type lamins to LBR localization and dynamics.

Nucleus (Austin, Tex.)·2026
See all related articles

Related Experiment Video

Updated: May 29, 2026

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Coordinating postmitotic nuclear pore complex assembly with abscission timing.

Douglas R Mackay1, Katharine S Ullman

  • 1Department of Oncological Sciences, Huntsman Cancer Institute, University of Utah, Salt Lake City, UT, USA.

Nucleus (Austin, Tex.)
|September 24, 2011
PubMed
Summary
This summary is machine-generated.

Disrupting nuclear pore complex assembly delays cell division by mislocalizing Aurora B kinase. This suggests a checkpoint ensuring nuclear reformation before cell abscission.

More Related Videos

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

Time-lapse Imaging of Mitosis After siRNA Transfection
08:21

Time-lapse Imaging of Mitosis After siRNA Transfection

Published on: June 6, 2010

Related Experiment Videos

Last Updated: May 29, 2026

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast
11:19

Spatiotemporal Analysis of Cytokinetic Events in Fission Yeast

Published on: February 20, 2017

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy
12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

Time-lapse Imaging of Mitosis After siRNA Transfection
08:21

Time-lapse Imaging of Mitosis After siRNA Transfection

Published on: June 6, 2010

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Cell division requires precise coordination of DNA segregation, nuclear reformation, and cytokinesis/abscission.
  • The mechanisms coordinating these overlapping events remain largely unknown.
  • Nuclear pore complex (NPC) assembly is critical for nuclear envelope reformation post-mitosis.

Purpose of the Study:

  • To investigate the coordination between nuclear pore complex assembly and cell abscission.
  • To elucidate the role of Aurora B kinase in this coordination.
  • To explore a potential abscission checkpoint linked to nuclear architecture.

Main Methods:

  • Cellular and molecular biology techniques were used to disrupt NPC assembly.
  • Immunofluorescence microscopy was employed to visualize protein localization and activity.
  • Analysis of abscission timing and Aurora B kinase activity in response to NPC assembly disruption.

Main Results:

  • Disruption of postmitotic NPC assembly triggers a delay in abscission.
  • This delay is dependent on Aurora B kinase activity.
  • Aberrantly active Aurora B kinase is mislocalized to the midbody during the delayed abscission phase.

Conclusions:

  • A novel Aurora B-mediated abscission checkpoint surveils NPC formation.
  • This checkpoint ensures complete nuclear reformation before daughter cell separation.
  • Nuclear architecture integrity is monitored to guarantee accurate cell division.