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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: May 29, 2026

Tracking Mouse Bone Marrow Monocytes In Vivo

Tracking Mouse Bone Marrow Monocytes In Vivo

Published on: February 27, 2015

Noninvasive cell-tracking methods.

Moritz F Kircher¹, Sanjiv S Gambhir, Jan Grimm

¹Department of Radiology, Memorial Sloan-Kettering Cancer Center, New York, NY 10065, USA.

Nature Reviews. Clinical Oncology

|September 28, 2011

Summary

This summary is machine-generated.

Cell-tracking methods are crucial for monitoring cell-based cancer therapies. These techniques visualize therapeutic cells in vivo, aiding in treatment development and understanding biological mechanisms.

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Last Updated: May 29, 2026

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Optimized Staining and Proliferation Modeling Methods for Cell Division Monitoring using Cell Tracking Dyes

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Area of Science:

Oncology
Biomedical Imaging
Cell Biology

Background:

Cell-based therapies, including adoptive immunotherapy and stem-cell therapy, are promising cancer treatments.
Effective therapeutic strategies require in vivo monitoring of cell location, distribution, viability, and fate.
Noninvasive imaging combined with cell-labeling is essential for real-time visualization and quantification.

Purpose of the Study:

To review the principles and approaches of cell-tracking methods.
To highlight applications of cell tracking in cancer research and clinical practice.
To emphasize the role of cell tracking in advancing cell-based cancer therapies.

Main Methods:

Exogenous cell labeling
Reporter gene transfection
Noninvasive imaging modalities (e.g., MRI, PET, optical imaging)

Main Results:

Cell-tracking enables real-time in vivo visualization of therapeutic cells.
Methods allow monitoring of cell distribution, accumulation, and function.
Tracking provides insights into cell fate, including activation and differentiation.

Conclusions:

Cell-tracking is indispensable for developing and evaluating cell-based cancer therapies.
These methods are vital for understanding tumor biology and treatment efficacy.
Applications span from preclinical animal models to human clinical trials.