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Updated: May 29, 2026

Expression and Purification of Virus-like Particles for Vaccination

Expression and Purification of Virus-like Particles for Vaccination

Published on: June 2, 2016

Protein delivery using engineered virus-like particles.

Stanislaw J Kaczmarczyk¹, Kalavathy Sitaraman, Howard A Young

¹Protein Expression Laboratory, Advanced Technology Program, Science Applications International Corporation-Frederick, Inc, Frederick, MD 21702, USA.

Proceedings of the National Academy of Sciences of the United States of America

|September 28, 2011

Summary

This summary is machine-generated.

Researchers developed virus-like particles (VLPs) from avian retroviruses as a safe protein delivery system. These adaptable VLPs overcome limitations of current methods, enabling efficient intracellular and surface protein delivery with demonstrated activity in cells.

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Area of Science:

Biotechnology
Molecular Biology
Cell Biology

Background:

Existing macromolecule delivery methods face challenges like inefficient uptake and endosomal entrapment.
Retroviral vectors for gene delivery pose risks due to host genome integration.

Purpose of the Study:

To develop a safe and adaptable alternative for protein delivery into living cells.
To demonstrate the efficacy of virus-like particles (VLPs) for intracellular and surface protein delivery.

Main Methods:

Utilized virus-like particles (VLPs) derived from avian retroviruses.
Engineered VLPs for intracellular delivery via Gag fusion proteins (e.g., Gag-Cre recombinase).
Engineered VLPs for surface display of proteins (e.g., murine IFN-γ, human TNF-related apoptosis-inducing ligand).

Main Results:

Demonstrated functional activity of encapsidated proteins (Gag-Cre, Gag-Fcy::Fur, Gag-human caspase-8) within recipient cells.
Showcased successful surface display of bioactive proteins on VLPs.
Confirmed VLP-mediated cellular responses, including STAT1 phosphorylation and induction of cell death.

Conclusions:

Avian retrovirus-derived VLPs offer a versatile and safe platform for protein delivery.
VLPs can be engineered for both intracellular and surface protein delivery, overcoming limitations of existing methods.
This VLP system shows promise for various biotechnological and therapeutic applications.