Related Experiment Video
Updated: May 29, 2026

Determination of the Gas-phase Acidities of Oligopeptides
Published on: June 24, 2013
A new ion source and procedures for atmospheric pressure-electron capture dissociation of peptides
Damon B Robb1, Jason C Rogalski, Juergen Kast
1Department of Chemistry, University of British Columbia, Vancouver, BC, V6T 1Z1, Canada. robb@chem.ubc.ca
Abstract:
We introduce a new atmospheric pressure-electron capture dissociation (AP-ECD) source in which conventional nanospray emitters are coupled with the source block and photoionization lamp of a PhotoSpray APPI source. We also introduce procedures for data collection and processing, aimed at maximizing the signal-to-background ratio of ECD products. Representative data from Substance P are presented to demonstrate the performance of the technique. Further, we demonstrate the effects of two important experimental variables, source temperature and vacuum-interface declustering potential (DP), on the method. Last, we show that even when a high source temperature is used to maximize efficiency, AP-ECD fragments of a model phosphorylated peptide retain the modification.
Related Concept Videos
Chemical Ionization (CI) Mass Spectrometry
Tandem Mass Spectrometry
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview
Peptide Identification Using Tandem Mass Spectrometry
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
Mass Spectrometers
