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Related Concept Videos

Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Regulated mRNA Transport02:22

Regulated mRNA Transport

In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing specific...

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Related Experiment Video

Updated: May 29, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Transcriptome analysis.

Frank Stahl1, Bernd Hitzmann, Kai Mutz

  • 1Institute for Technical Chemistry, Leibniz University, Hannover, Germany. stahl@iftc.uni-hannover.de

Advances in Biochemical Engineering/Biotechnology
|September 29, 2011
PubMed
Summary
This summary is machine-generated.

Transcriptome analysis, using methods like microarray and next-generation sequencing, optimizes mammalian cell cultures for better growth and protein production. This knowledge aids in improving cell lines through genome engineering for enhanced biopharmaceutical manufacturing.

More Related Videos

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Related Experiment Videos

Last Updated: May 29, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

Transcriptome Analysis of Single Cells
07:27

Transcriptome Analysis of Single Cells

Published on: April 25, 2011

Area of Science:

  • Systems Biology
  • Biotechnology
  • Molecular Biology

Background:

  • Mammalian cell cultures are crucial for producing recombinant proteins.
  • Optimizing cell growth rates and productivity is essential for biopharmaceutical manufacturing.
  • Understanding gene expression is key to improving cell line performance.

Purpose of the Study:

  • To review transcriptome analysis technologies for mammalian cell culture optimization.
  • To discuss the application of these technologies in genome engineering for cell line improvement.
  • To highlight the impact of transcriptome analysis on enhancing cell culture productivity.

Main Methods:

  • Detailed presentation of microarray technology for transcriptome analysis.
  • Explanation of next-generation sequencing (NGS) for comprehensive gene expression profiling.
  • Brief overview of protein-based analysis methods.

Main Results:

  • Transcriptome analysis provides critical insights into gene expression under specific culture conditions.
  • These insights enable targeted genome engineering strategies for cell line enhancement.
  • The discussed technologies significantly impact the optimization of mammalian cell cultures.

Conclusions:

  • Transcriptome analysis is a vital systems biology tool for mammalian cell culture development.
  • Microarray and next-generation sequencing are powerful techniques for understanding gene expression.
  • These methods facilitate the improvement of cell lines, leading to increased productivity in biomanufacturing.