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Updated: May 29, 2026

Peptide and Protein Quantification Using Automated Immuno-MALDI (iMALDI)
Published on: August 18, 2017
Mass spectrometric immunoassay revisited
Randall W Nelson1, Chad R Borges
1Molecular Biomarkers, The Biodesign Institute at Arizona State University, Tempe, AZ 85287, USA. randal.nelson@asu.edu
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) advancements enable new biological analyses. Mass Spectrometric Immunoassay offers a method to detect and quantify proteins in biological samples.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Biotechnology
Background:
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) has undergone significant development.
- These advancements, partly due to Dr. Marvin Vestal's contributions, have expanded its use in biological sciences.
- Mass Spectrometric Immunoassay is an analytical approach leveraging these improvements.
Purpose of the Study:
- To revisit the foundational concepts of Mass Spectrometric Immunoassay.
- To highlight its utility in detecting and quantifying proteins within biological samples.
Main Methods:
- Utilizing advancements in MALDI-TOFMS technology.
- Applying immunoassay principles for targeted protein detection.
Main Results:
- The study revisits the conceptual framework of Mass Spectrometric Immunoassay.
- It underscores the capability of this method for selective protein detection and quantification.
Conclusions:
- Improved MALDI-TOFMS technology facilitates sophisticated biological assays.
- Mass Spectrometric Immunoassay is a viable technique for protein analysis in biological contexts.
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