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Related Concept Videos

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
Next-generation Sequencing03:00

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features.
Ribosome Profiling02:24

Ribosome Profiling

Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
RACE - Rapid Amplification of cDNA Ends02:35

RACE - Rapid Amplification of cDNA Ends

Rapid Amplification of cDNA Ends, or RACE, is one of the most effective methods to obtain a full-length cDNA from an mRNA sequence between a known internal region to the unknown sequence at the 5’ or 3’ end. The unknown region is cloned in the cDNA by a gene-specific primer that binds the known end, and a hybrid primer that attaches a predefined anchor sequence to the unknown end of the cDNA. The sequence in between is amplified by PCR with an anchor primer and a gene-specific primer.
Since the...

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Related Experiment Video

Updated: May 29, 2026

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

Single-molecule direct RNA sequencing without cDNA synthesis.

Fatih Ozsolak1, Patrice M Milos

  • 1Helicos BioSciences Corporation, Cambridge, MA, USA. fozsolak@helicosbio.com

Wiley Interdisciplinary Reviews. RNA
|September 30, 2011
PubMed
Summary
This summary is machine-generated.

Direct RNA sequencing (DRS) offers a bias-free method for transcriptome analysis, bypassing complementary DNA conversion. This technology provides a more accurate view of RNA molecules for research and diagnostics.

More Related Videos

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

Related Experiment Videos

Last Updated: May 29, 2026

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications
05:41

2D-HELS MS Seq: A General LC-MS-Based Method for Direct and de novo Sequencing of RNA Mixtures with Different Nucleotide Modifications

Published on: July 10, 2020

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing
14:15

Highly Efficient Ligation of Small RNA Molecules for MicroRNA Quantitation by High-Throughput Sequencing

Published on: November 18, 2014

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • Transcriptome characterization is crucial for understanding cellular functions and diseases.
  • Current RNA analysis methods, like microarrays and next-generation sequencing, involve complementary DNA conversion, introducing biases.
  • Direct RNA sequencing (DRS) technology aims to overcome these limitations for more accurate transcriptome profiling.

Purpose of the Study:

  • To review the principles of single-molecule direct RNA sequencing (DRS).
  • To discuss sample preparation techniques for DRS.
  • To explore current and emerging research applications of DRS.

Main Methods:

  • Review of existing literature on direct RNA sequencing.
  • Explanation of the underlying technological principles of DRS.
  • Discussion of sample preparation workflows for DRS.

Main Results:

  • DRS enables direct measurement of RNA molecules, avoiding complementary DNA conversion biases.
  • The technology offers high sensitivity and the ability to detect RNA modifications.
  • DRS applications are expanding in research and clinical diagnostics.

Conclusions:

  • DRS represents a significant advancement in transcriptome analysis.
  • The technology provides a more accurate and comprehensive view of the transcriptome.
  • DRS holds great promise for future biological research and clinical applications.