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Updated: May 29, 2026

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Published on: June 30, 2022
Functions and mechanisms of spliceosomal small nuclear RNA pseudouridylation
Guowei Wu1, Andrew T Yu, Athena Kantartzis
1Department of Biochemistry and Biophysics, University of Rochester Medical Center, Rochester, NY, USA.
Abstract:
Pseudouridines are the most abundant and highly conserved modified nucleotides identified in spliceosomal small nuclear RNAs (snRNAs). Most pseudouridines are also clustered in functionally important regions of spliceosomal snRNAs. Experiments carried out in several independent experimental systems show that the pseudouridines in spliceosomal snRNAs are functionally important for pre-messenger RNA (mRNA) splicing. Experimental data also indicate that spliceosomal snRNA pseudouridylation can be catalyzed by both RNA-dependent (box H/ACA Ribonucleoproteins) and RNA-independent (protein-only enzymes) mechanisms.
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