Related Experiment Video
Updated: May 29, 2026

Iridium(III) Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
Published on: July 7, 2015
Luminescent Ir(III) complex exclusively made of polypyridine ligands capable of intercalating into calf-thymus DNA
Sebastiano Campagna1, Marco Cavazzini, Matteo Cusumano
1Dipartimento di Chimica Inorganica, Chimica Analitica e Chimica Fisica, Università di Messina, Via F. Stagno D'Alcontres 31, 98166 Messina, Italy. campagna@unime.it
Abstract:
Efficient intercalation of a luminescent Ir(III) complex exclusively made of polypyridine ligands in natural and synthetic biopolymers is reported for the first time. The emission of the complex is largely enhanced in the presence of [poly(dA-dT)(2)] and strongly quenched in the presence of [poly(dG-dC)(2)]. By comparing the emission decays in DNA and in synthetic polynucleotides, it is proposed that the emission quenching of the title compound by guanine residues in DNA is no longer effective over a distance of four dA-dT base pairs.
Related Concept Videos
Photoluminescence: Applications
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

