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Production of Autologous Platelet-Rich Plasma for Boosting In Vitro Human Fibroblast Expansion
Published on: February 24, 2021
Biologic filler using human fibroblasts and placenta extracts.
Eun Jung Oh1, Taek Kyun Kim, Jun Ho Shin
1Department of Plastic and Reconstructive Surgery, School of Medicine, Kyungpook National University, Daegu, South Korea.
The Journal of Craniofacial Surgery
|October 1, 2011
Summary
This study developed a novel biologic filler using human fibroblasts and placenta extract for soft tissue augmentation. The enhanced filler significantly improved volume maintenance and promoted extracellular matrix production over eight weeks.
Area of Science:
- Regenerative Medicine
- Biomaterials Science
- Plastic and Reconstructive Surgery
Background:
- Soft tissue augmentation faces challenges with current injectable fillers, including adverse effects and absorption.
- Existing fillers can cause inflammation, distortion, and necessitate repeat procedures.
- Cell therapy offers a potential solution for more effective and durable soft tissue augmentation.
Purpose of the Study:
- To develop and evaluate a novel biologic filler for soft tissue augmentation using human fibroblasts and placenta extracts.
- To assess the efficacy of the biologic filler in terms of volume maintenance and tissue regeneration.
- To compare the effects of different culture conditions on the performance of the biologic filler.
Main Methods:
- An in vivo study involving 40 nude mice divided into control and three experimental groups.
- Injection of cultured human fibroblasts with fibrin glue, with experimental groups receiving placenta extract and/or fetal bovine serum.
- Evaluation over 8 weeks using gross, histologic, and biomolecular analyses, including assays for collagen and glycosaminoglycan, and gene expression analysis for COL1A1.
Main Results:
- Experimental groups showed significantly better geometric maintenance compared to the control group, with group 3 (placenta extract + fetal bovine serum) demonstrating the greatest volume reservation.
- Histological analysis revealed abundant fibroblast proliferation and extracellular matrix (collagen, glycosaminoglycan) deposition in experimental groups.
- Biomolecular analysis confirmed significant differences in collagen and glycosaminoglycan levels and COL1A1 gene expression between experimental and control groups.
Conclusions:
- The developed biologic filler, particularly when combining human fibroblasts with placenta extract and fetal bovine serum, is effective for soft tissue augmentation.
- This cell therapy approach promotes fibroblast proliferation and extracellular matrix synthesis, leading to improved volume maintenance and tissue regeneration.
- The findings suggest a promising new direction for addressing limitations of current soft tissue fillers in plastic and reconstructive surgery.
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