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Fabrication of Refractive-index-matched Devices for Biomedical Microfluidics
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High refractive index silicone gels for simultaneous total internal reflection fluorescence and traction force

Edgar Gutierrez1, Eugene Tkachenko, Achim Besser

  • 1Department of Physics, University of California San Diego, La Jolla, California, United States of America.

Plos One
|October 1, 2011
PubMed

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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.