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Isolation and biological characterization of an adenovirus of rhesus macaques
Abstract:
The etiology of a disease in rhesus monkeys the main clinical manifestation of which was acute conjunctivitis of an epizootic character has been studied. The cytopathogenic agent well propagating in primarily trypsinized kidney cells of monkeys has been isolated when investigating the affected eye mucosa. It was not pathogenic for laboratory animals. The mean diameter of the virions is 75 nm, the buoyant density in CsCl is 1.34 g/cm3, the viral DNA density is 1.706 g/cm3. The biological properties and findings of physicochemical, electron-microscopic, and serologic investigations allow one to allocate the isolated agent to the SV-37 strain, a representative of the adenovirus group.
Insights
Researchers identified a novel adenovirus (SV-37 strain) causing acute conjunctivitis in rhesus monkeys. This virus, isolated from eye mucosa, showed specific characteristics and was not pathogenic to other lab animals.
Area of Science:
- Virology
- Primate Pathology
- Ophthalmology
Background:
- Epizootic acute conjunctivitis outbreaks pose a significant health concern in rhesus monkey populations.
- Understanding the etiology of such diseases is crucial for effective disease management and prevention in non-human primates.
Purpose of the Study:
- To investigate the causative agent of acute conjunctivitis in rhesus monkeys.
- To characterize the isolated viral agent using various laboratory techniques.
Main Methods:
- Isolation of a cytopathogenic agent from affected rhesus monkey eye mucosa.
- Propagation of the agent in primary trypsinized monkey kidney cells.
- Physicochemical, electron-microscopic, and serologic characterization of the virions.
- Pathogenicity testing in laboratory animals.
Main Results:
- An agent was isolated from rhesus monkeys with acute conjunctivitis.
- The agent propagated well in monkey kidney cells but was not pathogenic in laboratory animals.
- Physicochemical analysis revealed virion diameter (75 nm), buoyant density (1.34 g/cm³), and viral DNA density (1.706 g/cm³).
- Serologic and biological properties identified the agent as the SV-37 strain, an adenovirus.
Conclusions:
- The isolated SV-37 strain is identified as the causative agent of epizootic acute conjunctivitis in rhesus monkeys.
- The findings contribute to the understanding of adenovirus-related diseases in non-human primates.
- This research aids in the diagnosis and control of conjunctivitis outbreaks in captive monkey populations.