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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...

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Related Experiment Video

Updated: May 28, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Surface plasmon resonance for proteomics.

Nico J de Mol1

  • 1Department of Pharmaceutical Sciences, Utrecht University, Utrecht, The Netherlands. N.J.deMol@uu.nl

Methods in Molecular Biology (Clifton, N.J.)
|October 4, 2011
PubMed
Summary
This summary is machine-generated.

Surface Plasmon Resonance (SPR) is a label-free method for biomolecular analysis. Integrating SPR with mass spectrometry (MS) enhances proteomics by identifying captured molecules, enabling high-throughput analysis of microarrays.

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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Related Experiment Videos

Last Updated: May 28, 2026

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
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Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Proteomics

Background:

  • Surface Plasmon Resonance (SPR) is a label-free technique proven for biomolecular interaction analysis, including affinity and kinetics.
  • The growing field of proteomics demands high-throughput analysis, driving the development of SPR systems for microarrays.

Purpose of the Study:

  • To review recent advancements in applying Surface Plasmon Resonance (SPR) to proteomics.
  • To highlight the integration of SPR with mass spectrometry (MS) for enhanced molecular identification.

Main Methods:

  • Utilizing SPR imaging for multiplexed array analysis of binding reactions.
  • Integrating SPR with mass spectrometric (MS) techniques, including MALDI-TOF MS and LC-MS/MS.
  • Exploring on-chip MALDI-TOF MS for arrayed spots on SPR surfaces.

Main Results:

  • SPR systems are evolving for high-throughput microarray analysis in proteomics.
  • Integration with MS allows for the identification of molecules captured on SPR surfaces.
  • On-chip MALDI-TOF MS is a promising approach compatible with SPR arrays.

Conclusions:

  • The combination of SPR and MS offers powerful solutions for modern proteomics.
  • Emerging and upcoming instruments are expanding the capabilities of SPR in proteomic research.
  • These integrated techniques facilitate detailed analysis of biomolecular interactions and captured analytes.