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Updated: May 28, 2026
11:26
Assessment of Dopaminergic Homeostasis in Mice by Use of High-performance Liquid Chromatography Analysis and Synaptosomal Dopamine Uptake
Published on: September 21, 2017
Dopamine 2 (DA-2) receptor assays using sympathetic nerve terminals.
1University of Iowa, Iowa City, Iowa, USA.
Current Protocols in Pharmacology
|October 4, 2011
Summary
This study details methods for measuring dopamine-2 receptor activity using isolated atria. These assays quantify how compounds affect norepinephrine release, impacting physiological responses.
Area of Science:
- Pharmacology
- Neuroscience
- Cardiovascular Physiology
Background:
- Dopamine-2 (DA-2) receptors modulate norepinephrine release from sympathetic nerves.
- Activation of DA-2 receptors leads to physiological effects like bradycardia and hypotension.
Purpose of the Study:
- To describe in vitro methods for quantifying DA-2 receptor stimulating activity.
- To detail tissue preparation and receptor assays for isolated atria.
Main Methods:
- Utilizing isolated cat and guinea pig atria.
- Employing field stimulation and superfusion techniques.
- Performing dopamine receptor assays.
Main Results:
- Established protocols for measuring DA-2 receptor agonist activity.
- Demonstrated the utility of isolated atria in receptor pharmacology.
Conclusions:
- Isolated atria preparations provide a reliable system for assessing DA-2 receptor activity.
- These methods can quantify compound effects on norepinephrine release and associated physiological changes.
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