Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

Two-Dimensional Microscopy in Microbiology

Two-Dimensional Microscopy in Microbiology

Two-dimensional (2D) microscopy encompasses a range of optical techniques that capture images within a single focal plane, offering detailed representations of microscopic structures. These techniques are essential in biological and medical research, enabling the visualization of cellular and subcellular structures with different levels of contrast and specificity.There are several major types of 2D microscopy, each with strengths and applications.Bright-Field MicroscopyBright-field microscopy...

Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

Overview of Microscopy Techniques

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...

Immunofluorescence Microscopy

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Formulation, Development, and Evaluation of Herbal Effervescent Mouthwash Tablet Containing <i>Azadirachta Indica</i> (Neem) and Curcumin for the Maintenance of Oral Hygiene.

Recent patents on drug delivery & formulation·2020

Same author

Ameliorative effect of curcumin on altered expression of CACNA1A and GABRD in the pathogenesis of FeCl<sub>3</sub>-induced epilepsy.

Molecular biology reports·2020

Same author

Echocardiographic assessment of hemodynamic changes in preterm neonates with shock: a prospective pragmatic cohort study.

European journal of pediatrics·2020

Same author

Plethysmography variability index (PVI) changes in preterm neonates with shock-an observational study.

European journal of pediatrics·2020

Same author

Viral Evolution will Automatically Resolve COVID-19 Pandemic.

Combinatorial chemistry & high throughput screening·2020

Same author

Letter by Sharma and Rasmussen Regarding Article, "Mechanical Thrombectomy in the Era of the COVID-19 Pandemic: Emergency Preparedness for Neuroscience Teams: A Guidance Statement From the Society of Vascular and Interventional Neurology".

Stroke·2020

Same journal

Detection and Sorting of Extracellular Vesicles and Viruses Using nanoFACS.

Current protocols in cytometry·2020

Same journal

Live Imaging of the Lung.

Current protocols in cytometry·2020

Same journal

Small Particle Fluorescence and Light Scatter Calibration Using FCM<sub>PASS</sub> Software.

Current protocols in cytometry·2020

Same journal

Optimized Stochastic Optical Reconstruction Microscopy for Imaging Chromatin Structure in Pathological Tissue.

Current protocols in cytometry·2020

Same journal

Flow Cytometric Quantification of Granulocytic Alkaline Phosphatase Activity in Unlysed Whole Blood.

Current protocols in cytometry·2020

Same journal

Practical Guidelines for Collection, Manipulation and Inactivation of SARS-CoV-2 and COVID-19 Clinical Specimens.

Current protocols in cytometry·2020

See all related articles

Search research articles

Related Experiment Video

Updated: May 28, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Light microscopy digital imaging.

James Joubert¹, Deepak Sharma¹

¹Photometrics, Tucson, Arizona.

Current Protocols in Cytometry

|October 4, 2011

Summary

This summary is machine-generated.

This guide covers digital imaging hardware for light microscopy, focusing on CMOS, CCD, and EMCCD sensors. Understanding their pros and cons helps select the right sensor for specific microscopy applications.

More Related Videos

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Published on: March 24, 2011

Related Experiment Videos

Last Updated: May 28, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Published on: March 24, 2011

Area of Science:

Microscopy
Digital Imaging
Optical Instrumentation

Background:

Overview of digital imaging hardware essential for modern light microscopy.
Introduction to primary image sensor technologies: CMOS, CCD, and EMCCD.

Observation:

Detailed review of sensor architectures and formats.
Analysis of the strengths and weaknesses of CMOS, CCD, and EMCCD sensors.
Examination of color camera design strategies.

Findings:

Sensor selection is dictated by specific application requirements based on performance characteristics.
Understanding sensor limitations is crucial for optimal image acquisition in microscopy.

Implications:

Informed selection of digital imaging hardware enhances microscopy research outcomes.
This knowledge aids in optimizing imaging protocols and troubleshooting hardware issues.