Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl hydroxylase and factor...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
Epigenetic Regulation01:37

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
Epigenetic Regulation01:46

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Environmental ubiquity but limited host taxonomic distribution of co-occurring metal(loid)-resistance genes and persistent organic pollutant-transformation genes in global inland waters.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Intrinsic coordination of dynamic molecular signatures shape the human prefrontal cortex.

bioRxiv : the preprint server for biology·2026
Same author

Environmentally relevant concentrations of nanoplastic increase the accumulation and endocrine effects of BDE47 and its metabolite 6-OH-BDE-47 in male frogs' testes.

Journal of environmental sciences (China)·2026
Same author

A Framework to Decipher and Report Implementation Strategies and Pragmatic Attributes of Trial Design Domains From Pre-Implementation Studies Identified From a Systematic Review: A Methodological Study.

Journal of evaluation in clinical practice·2026
Same author

The Role of Gut Microbiota in Fish Viral Infection: Mechanisms and Microbiota-Targeted Interventions.

Aquaculture nutrition·2026
Same author

Sulfamethoxazole at environmental concentrations affects PPARα-mediated lipid metabolism in male frogs.

Journal of advanced research·2026
Same journal

Sinomenine Regulates the TRIM32/IRF1/TRAF6 Axis to Inhibit Pyroptosis in Atopic Dermatitis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

Urolithin A Mitigates Renal Fibrosis by Promoting Fatty Acid Oxidation Through Orchestrating β-Catenin Signaling.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

MiR-124 Inhibits Lipid Deposition in Mouse Liver by Targeting the Trib3/Hnf4α Pathway.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

TCN2 Drives Psoriasis-Like Inflammation and Keratinocyte Hyperproliferation, Correlating With IL-1β and STAT3 Activation.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

The Synthetic Melanocortin Agonist NDP-MSH Ameliorates THSD7A-Associated Membranous Nephropathy in an Active Immunization Mouse Model.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
Same journal

Brain-Derived Neurotrophic Factor and Associated Signaling in Kidney Diseases.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2026
See all related articles

Related Experiment Video

Updated: May 28, 2026

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

Origin-specific epigenetic program correlates with vascular bed-specific differences in Rgs5 expression.

Hangjun Zhang1, Steven Gu, Basil Al-Sabeq

  • 1Department of Physiology, Heart, and Stroke, University of Toronto, Toronto, ON Canada.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|October 4, 2011
PubMed
Summary
This summary is machine-generated.

Vascular smooth muscle cell (VSMC) Rgs5 expression differences arise from developmental origin, not location. Epigenetic changes, specifically DNA methylation, regulate these VSMC gene expression patterns during maturation.

More Related Videos

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)
11:19

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)

Published on: September 26, 2015

Related Experiment Videos

Last Updated: May 28, 2026

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo
09:53

Whole-mount Immunohistochemical Analysis for Embryonic Limb Skin Vasculature: a Model System to Study Vascular Branching Morphogenesis in Embryo

Published on: May 20, 2011

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues
13:03

Epigenetic Regulation of Cardiac Differentiation of Embryonic Stem Cells and Tissues

Published on: June 3, 2016

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)
11:19

Correlating Gene-specific DNA Methylation Changes with Expression and Transcriptional Activity of Astrocytic KCNJ10 (Kir4.1)

Published on: September 26, 2015

Area of Science:

  • Developmental Biology
  • Epigenetics
  • Vascular Biology

Background:

  • Vascular smooth muscle cell (VSMC) heterogeneity is linked to developmental origin.
  • Mechanisms driving VSMC phenotypic differences remain unclear.
  • Rgs5 expression varies significantly between different vascular beds during maturation.

Purpose of the Study:

  • Investigate mechanisms controlling vascular bed-specific Rgs5 expression differences.
  • Determine if Rgs5 expression is linked to VSMC developmental origin.
  • Elucidate the role of epigenetics in regulating VSMC gene expression.

Main Methods:

  • Utilized Rgs5-LacZ reporter mice to track VSMC origin and Rgs5 expression.
  • Compared Rgs5 expression in VSMCs from different branchial arches and embryonic origins.
  • Analyzed Rgs5 promoter methylation in carotid arteries versus descending aortas.
  • Performed in vitro methylation assays to assess Rgs5 promoter activity.

Main Results:

  • Rgs5 expression was significantly higher in adult descending aortas compared to carotid arteries.
  • Rgs5 expression patterns correlated with VSMC developmental origin (e.g., splanchnic mesoderm, somites).
  • Increased Rgs5 promoter CpG methylation was observed in adult carotid arteries versus descending aortas.
  • In vitro Rgs5 promoter methylation reduced transcriptional activity.

Conclusions:

  • Vascular bed-specific Rgs5 expression is determined by VSMC developmental origin.
  • Epigenetic mechanisms, particularly DNA methylation, play a crucial role in regulating VSMC gene transcription.
  • An origin-dependent epigenetic program influences VSMC gene expression during maturation.