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Engineering laccases: in search for novel catalysts
Viviane Robert1, Yasmina Mekmouche, Pierre R Pailley
1Laboratoire Biosciences, Institut des Sciences Moléculaires de Marseille, Université Aix-Marseille, ISM2 CNRS UMR 6263, Marseille Cedex 20, France.
Current Genomics
|October 4, 2011
Summary
Laccases, a type of enzyme, are being engineered for diverse applications. Our lab focuses on designing novel biocatalysts using laccases through protein modification techniques.
Area of Science:
- Biochemistry
- Enzymology
- Biotechnology
Background:
- Laccases (p-diphenol oxidase, EC 1.10.3.2) are multicopper oxidases catalyzing redox reactions.
- Discovered over a century ago, laccases from various sources (plants, fungi, bacteria) have been extensively studied.
- These enzymes oxidize organic substrates using dioxygen, reducing it to water.
Purpose of the Study:
- To provide an overview of strategies and results in designing novel biocatalysts based on laccases.
- To highlight the potential of laccases in various applications through protein engineering.
Main Methods:
- Utilizing molecular evolution techniques.
- Employing protein modification strategies.
- Designing and developing new laccase-based biocatalysts.
Main Results:
- Demonstrated successful application of protein modification techniques for laccase engineering.
- Developed tailored laccases with potential for specific applications.
- Showcased laboratory strategies for biocatalyst design.
Conclusions:
- Laccase engineering holds significant promise for developing advanced biocatalysts.
- Tailored laccases can be applied in areas such as drug synthesis, biosensors, and biofuel cells.
- Protein modification techniques are powerful tools for advancing laccase applications.
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