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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
Development and evaluation of a selective medium for Brucella suis
Ana Cristina Ferreira1, Cláudia Almendra, Regina Cardoso
1Instituto Nacional de Recursos Biológicos (INRB), I.P./LNIV, Laboratório Nacional de Investigação Veterinária, Estrada de Benfica, 701, 1549-011 Lisboa, Portugal. Cristina.ferreira@lniv.min-agricultura.pt
Research in Veterinary Science
|October 5, 2011
Summary
A novel selective medium, LNIV-M, effectively isolates Brucella suis from wild boar tissues. This new medium demonstrates superior diagnostic performance compared to existing methods for Brucella suis detection.
Area of Science:
- Veterinary Microbiology
- Infectious Diseases
- Diagnostic Bacteriology
Background:
- Brucella suis poses a significant threat to animal and public health.
- Current diagnostic methods for Brucella suis isolation have limitations.
- Development of a more effective selective medium is crucial for accurate diagnosis.
Purpose of the Study:
- To develop and evaluate a new selective medium, LNIV-M, for the isolation of Brucella suis.
- To assess the diagnostic performance of LNIV-M compared to established media.
- To determine the efficacy of LNIV-M in isolating Brucella suis from wild boar samples.
Main Methods:
- Evaluation of Brucella suis growth on different basal media and antibiotic susceptibility.
- Testing of LNIV-M, Farrell's, and modified Thayer-Martin media for Brucella suis isolation.
- Culturing of 1649 wild boar tissue samples using the three different media.
Main Results:
- LNIV-M demonstrated high diagnostic sensitivity, detecting 93.6% of positive animals and 62.6% of positive samples.
- LNIV-M outperformed Farrell's and modified Thayer-Martin media in detecting positive samples.
- A total of 139 samples from 63 wild boars tested positive for Brucella suis.
Conclusions:
- LNIV-M is a highly effective selective medium for the isolation of Brucella suis.
- The diagnostic performance of LNIV-M is adequate for detecting Brucella suis in wild boar populations.
- LNIV-M offers an improved tool for Brucella suis surveillance and control efforts.
