Related Experiment Video
Updated: May 28, 2026
Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
Published on: August 5, 2016
Development and evaluation of a selective medium for Brucella suis
Ana Cristina Ferreira1, Cláudia Almendra, Regina Cardoso
1Instituto Nacional de Recursos Biológicos (INRB), I.P./LNIV, Laboratório Nacional de Investigação Veterinária, Estrada de Benfica, 701, 1549-011 Lisboa, Portugal. Cristina.ferreira@lniv.min-agricultura.pt
Abstract:
A new selective medium, named LNIV-M, has been developed for isolation of Brucella suis. In this work, we evaluated the growth of B. suis reference and field strains from domestic pigs in different basal media and the susceptibility to different antibiotics contained in the currently used Farrell's and modified Thayer-Martin media. We also determined the efficacy of LNIV-M and its diagnostic performance for isolating B. suis from wild boar tissue samples. A total of 1649 samples from 918 hunter-harvested wild boars were cultured in LNIV-M, Farrell's and modified Thayer-Martin media. One hundred and thirty-nine (8.4%) samples from 63 (6.9%) animals resulted in a positive culture. LNIV-M detected 93.6% and 62.6% of positive animals and samples, respectively, while Farrell's and modified Thayer-Martin media detected, respectively, 92.1% and 79.4% of positive animals and 58.3% and 59.7% of samples. These results confirm the adequate diagnostic performance of LNIV-M in the isolation of B. suis.
Insights
A novel selective medium, LNIV-M, effectively isolates Brucella suis from wild boar tissues. This new medium demonstrates superior diagnostic performance compared to existing methods for Brucella suis detection.
Area of Science:
- Veterinary Microbiology
- Infectious Diseases
- Diagnostic Bacteriology
Background:
- Brucella suis poses a significant threat to animal and public health.
- Current diagnostic methods for Brucella suis isolation have limitations.
- Development of a more effective selective medium is crucial for accurate diagnosis.
Purpose of the Study:
- To develop and evaluate a new selective medium, LNIV-M, for the isolation of Brucella suis.
- To assess the diagnostic performance of LNIV-M compared to established media.
- To determine the efficacy of LNIV-M in isolating Brucella suis from wild boar samples.
Main Methods:
- Evaluation of Brucella suis growth on different basal media and antibiotic susceptibility.
- Testing of LNIV-M, Farrell's, and modified Thayer-Martin media for Brucella suis isolation.
- Culturing of 1649 wild boar tissue samples using the three different media.
Main Results:
- LNIV-M demonstrated high diagnostic sensitivity, detecting 93.6% of positive animals and 62.6% of positive samples.
- LNIV-M outperformed Farrell's and modified Thayer-Martin media in detecting positive samples.
- A total of 139 samples from 63 wild boars tested positive for Brucella suis.
Conclusions:
- LNIV-M is a highly effective selective medium for the isolation of Brucella suis.
- The diagnostic performance of LNIV-M is adequate for detecting Brucella suis in wild boar populations.
- LNIV-M offers an improved tool for Brucella suis surveillance and control efforts.
