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Related Experiment Video

Updated: May 28, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
15:29

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

Development and evaluation of a selective medium for Brucella suis.

Ana Cristina Ferreira1, Cláudia Almendra, Regina Cardoso

  • 1Instituto Nacional de Recursos Biológicos (INRB), I.P./LNIV, Laboratório Nacional de Investigação Veterinária, Estrada de Benfica, 701, 1549-011 Lisboa, Portugal. Cristina.ferreira@lniv.min-agricultura.pt

Research in Veterinary Science
|October 5, 2011
PubMed
Summary

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A novel selective medium, LNIV-M, effectively isolates Brucella suis from wild boar tissues. This new medium demonstrates superior diagnostic performance compared to existing methods for Brucella suis detection.

Area of Science:

  • Veterinary Microbiology
  • Infectious Diseases
  • Diagnostic Bacteriology

Background:

  • Brucella suis poses a significant threat to animal and public health.
  • Current diagnostic methods for Brucella suis isolation have limitations.
  • Development of a more effective selective medium is crucial for accurate diagnosis.

Purpose of the Study:

  • To develop and evaluate a new selective medium, LNIV-M, for the isolation of Brucella suis.
  • To assess the diagnostic performance of LNIV-M compared to established media.
  • To determine the efficacy of LNIV-M in isolating Brucella suis from wild boar samples.

Main Methods:

  • Evaluation of Brucella suis growth on different basal media and antibiotic susceptibility.
  • Testing of LNIV-M, Farrell's, and modified Thayer-Martin media for Brucella suis isolation.

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Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
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Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Published on: November 8, 2016

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Last Updated: May 28, 2026

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
15:29

Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells

Published on: August 5, 2016

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species
09:34

Development of a More Sensitive and Specific Chromogenic Agar Medium for the Detection of Vibrio parahaemolyticus and Other Vibrio Species

Published on: November 8, 2016

  • Culturing of 1649 wild boar tissue samples using the three different media.

    • Main Results:

    • LNIV-M demonstrated high diagnostic sensitivity, detecting 93.6% of positive animals and 62.6% of positive samples.
    • LNIV-M outperformed Farrell's and modified Thayer-Martin media in detecting positive samples.
    • A total of 139 samples from 63 wild boars tested positive for Brucella suis.

    Conclusions:

    • LNIV-M is a highly effective selective medium for the isolation of Brucella suis.
    • The diagnostic performance of LNIV-M is adequate for detecting Brucella suis in wild boar populations.
    • LNIV-M offers an improved tool for Brucella suis surveillance and control efforts.