Related Experiment Video
Updated: May 28, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
Published on: December 11, 2021
First steps for fluorescence correlation spectroscopy of living cells
Abstract:
Fluorescence correlation spectroscopy (FCS) has become a powerful and sensitive tool in biochemistry and biophysics. It directly obtains physical parameters such as the average number of fluorescent molecules and their diffusion time in a tiny detection area. It also provides other useful information such as the brightness of molecules. Ultimately, it can give precise information about molecular interactions in the aqueous condition. This article outlines the basic parameters and properties of FCS.
More Related Videos
08:43A Fluorescence Fluctuation Spectroscopy Assay of Protein-Protein Interactions at Cell-Cell Contacts
Published on: December 1, 2018
10:59Determination of Lipid Raft Partitioning of Fluorescently-tagged Probes in Living Cells by Fluorescence Correlation Spectroscopy (FCS)
Published on: April 6, 2012