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Updated: May 28, 2026

Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes
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Fluorescent in situ Hybridization on Mitotic Chromosomes of Mosquitoes

Published on: September 17, 2012

[Application and progress of fluorescence in situ hybridization in schistosome biology]

Xiao-Jin Mo1, Zheng Feng, Wei Hu

  • 1National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai 200025, China.

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi = Chinese Journal of Parasitology & Parasitic Diseases
|October 6, 2011
PubMed

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FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...