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Related Experiment Video

Updated: May 28, 2026

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
11:47

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Published on: June 8, 2014

Cell-based resorption assays for bone graft substitutes.

Ziyang Zhang1, José T Egaña, Ann K Reckhenrich

  • 1Klinik und Poliklinik für Plastische Chirurgie und Handchirurgie, Klinikum Rechts der Isar, Technische Universität München, München, Germany.

Acta Biomaterialia
|October 6, 2011
PubMed
Summary

This review updates on cell-based assays for assessing bone substitute resorption. These in vitro methods offer reproducible, animal-free evaluation of biomaterial degradation and bone integration.

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Regenerative Medicine

Background:

  • Clinical use of resorbable bone substitutes is increasing, demanding novel biomaterials.
  • Ideal substitutes must support bone remodeling through controlled resorption and replacement.
  • Assessing resorbability is crucial for predicting in vivo performance.

Purpose of the Study:

  • To provide an updated review of in vitro cell-based resorption assays for bone substitutes.
  • To compare different cell models and species used in these assays.
  • To discuss optical methods for analyzing resorption and compare cell-based assays with standard ISO degradation tests.

Main Methods:

  • Comparison of various cell types and species for osteoclast differentiation and bone substitute resorption.

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Last Updated: May 28, 2026

A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders
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A Novel in vivo Gene Transfer Technique and in vitro Cell Based Assays for the Study of Bone Loss in Musculoskeletal Disorders

Published on: June 8, 2014

Bone Conditioned Medium: Preparation and Bioassay
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Bone Conditioned Medium: Preparation and Bioassay

Published on: July 8, 2015

Biological Compatibility Profile on Biomaterials for Bone Regeneration
10:28

Biological Compatibility Profile on Biomaterials for Bone Regeneration

Published on: November 16, 2018

  • Description of 2D and 3D optical microscopy techniques for analyzing resorption pits.
  • Evaluation of limitations and advantages of cell-based assays versus ISO degradation assays.

    • Main Results:

    • Cell-based assays enable reproducible, cost-effective, and animal-free assessment of bone substitute resorption.
    • Advances in microscopy allow detailed analysis of biomaterial degradation.
    • Human osteoclast models are now feasible due to discoveries in osteoclast differentiation factors.

    Conclusions:

    • In vitro cell-based assays are valuable tools for evaluating bone substitute resorbability.
    • These assays complement or offer advantages over traditional in vivo or ISO degradation methods.
    • Further development and standardization of cell-based assays will enhance biomaterial development for bone regeneration.