Antioxidant activity of flavonoids evaluated with myoglobin method

Area of Science:

• Biochemistry
• Food Science
• Analytical Chemistry

Background:

• Reactive oxygen species (ROS) are implicated in cellular damage.
• Flavonoids and non-flavonoids are plant-derived compounds with known antioxidant properties.
• Accurate assessment of antioxidant activity is crucial for food science and biotechnology.

Purpose of the Study:

• To quantify the antioxidant activities of four flavonoids and two non-flavonoids against four types of ROS.
• To validate a myoglobin-based assay for measuring antioxidant capacity.
• To visualize and compare antioxidant profiles using cobweb charts.

Main Methods:

• Utilized a myoglobin method to measure antioxidant activity by monitoring myoglobin's reaction with ROS.
• Quantified antioxidant capacity using the Myoglobin Protective Ratio (MPR).
• Employed DPPH quenching assay to determine Vitamin C Equivalent Concentration and generated 5-axe cobweb charts for comprehensive evaluation.

Main Results:

• Four flavonoids (rutin, quercetin, luteolin, kaempferol) displayed similar antioxidant activity patterns.
• Two non-flavonoids (chlorogenic acid, pyrocatechol) showed distinct antioxidant profiles compared to flavonoids.
• The myoglobin method effectively differentiated antioxidant capacities and patterns.

Conclusions:

• The myoglobin method combined with cobweb charts provides a robust tool for evaluating antioxidant activities of plant-derived compounds.
• This approach is valuable for assessing antioxidant properties in plant-derived foods.
• The method can be adapted for monitoring antioxidant conditions in plant cell culture media.