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Updated: May 28, 2026

Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

Published on: December 1, 2021

High-speed multineuron calcium imaging using Nipkow-type confocal microscopy.

Naoya Takahashi¹, Shigeyuki Oba, Naoto Yukinawa

¹Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.

Current Protocols in Neuroscience

|October 6, 2011

Summary

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Nipkow-disk confocal microscopy enables faster neuronal activity monitoring. This technique allows functional multineuron calcium imaging at high speeds, capturing fast electrical events in hundreds of neurons.

Area of Science:

Neuroscience
Optical Microscopy
Cellular Imaging

Background:

Conventional confocal and two-photon microscopy use raster-scanning, limiting imaging speeds to tens of frames per second.
These speeds are insufficient for capturing rapid neuronal electrical events.
Nipkow-type spinning-disk confocal microscopy offers a solution using multiple laser beams.

Purpose of the Study:

To describe experimental procedures for functional multineuron calcium imaging (fMCI) using Nipkow-disk confocal microscopy.
To enable high-speed, large-scale monitoring of neuronal activity.

Main Methods:

Utilizing Nipkow-disk confocal microscopy with multiple laser beams.
Implementing functional multineuron calcium imaging (fMCI).
Achieving cellular resolution imaging.

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Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Published on: February 7, 2018

Related Experiment Videos

Last Updated: May 28, 2026

Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

Registration of Calcium Transients in Mouse Neuromuscular Junction with High Temporal Resolution using Confocal Microscopy

Published on: December 1, 2021

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Two-photon Calcium Imaging in Neuronal Dendrites in Brain Slices

Published on: March 15, 2018

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Ratiometric Calcium Imaging of Individual Neurons in Behaving Caenorhabditis Elegans

Published on: February 7, 2018

Main Results:

Monitoring the activity of hundreds of neurons simultaneously.
Achieving video rates of up to 2000 frames per second.
Capturing fast electrical events in neurons at high temporal resolution.

Conclusions:

Nipkow-disk confocal microscopy significantly enhances the speed of neuronal activity imaging.
This method allows for mass monitoring of neuronal populations at cellular resolution.
The technique is suitable for studying fast temporal patterns of neuronal electrical events.