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Method of applying internal standard to dried matrix spot samples for use in quantitative bioanalysis
Paul Abu-Rabie1, Philip Denniff, Neil Spooner
1Platform Technology and Science, Drug Metabolism and Pharmacokinetics, GlaxoSmithKline Research and Development, Ware, Hertfordshire, United Kingdom. Paul.2.Abu-Rabie@gsk.com
Analytical Chemistry
|October 7, 2011
Summary
A new TouchSpray technique effectively applies internal standards (IS) to dried blood spot (DBS) samples before extraction. This method ensures accurate and precise quantification for pharmacokinetic and toxicokinetic studies.
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Accurate quantification of pharmaceuticals in biological samples is crucial for pharmacokinetic and toxicokinetic studies.
- Applying internal standards (IS) to dried matrix spot (DMS) samples before extraction presents a technical challenge.
- Existing methods for IS application may not ensure optimal integration with the sample matrix.
Purpose of the Study:
- To introduce and evaluate a novel technique for applying internal standards (IS) to dried blood spot (DBS) samples.
- To assess the feasibility of using TouchSpray technology for IS application in quantitative bioanalysis.
- To compare the accuracy and precision of the novel IS application method against traditional approaches.
Main Methods:
- A piezo electric spray system (TouchSpray) was utilized to apply methanol containing IS to DBS samples.
- Three methods of IS application were compared: pre-spotting into blood (control 1), addition to extraction solvent (control 2), and TouchSpray application (test).
- Quantitative analysis was performed using both manual extraction and direct elution techniques.
Main Results:
- The TouchSpray technique demonstrated comparable accuracy and precision to conventional methods for IS application.
- No significant differences in accuracy and precision were observed between the three tested IS application methods.
- The study confirmed the potential of the TouchSpray method for practical application in quantitative bioanalysis.
Conclusions:
- The TouchSpray technology offers a viable and effective method for applying internal standards to DBS samples prior to extraction.
- This novel approach facilitates improved sample preparation workflows for pharmaceutical analysis in toxicokinetic and pharmacokinetic studies.
- The findings support the integration of TouchSpray technology for robust and reliable quantitative determination of circulating drug exposures.
