An in vitro Survey on the Apoptotic Effects of UVB Ray in Bacillus anthracis
1MSc, Microbiology Department, Faculty of Basic Sciences, Islamic Azad University, Shahr-e-Qods Branch, Tehran - Iran.
Objectives:
Bacillus anthracis as the bacterial agent of anthrax, is spread in different geographical zones around the world.The purpose of this survey was to observe eventual apoptotic reactions in total genomic DNA of UVB irradiated colonies of Bacillus anthracis.
Materials And Methods:
The colonies of Bacillus anthracis were exposed to UVB ray for 10 minutes; then, the DNA molecules of control and irradiated colonies were extracted. Finally, the DNA samples mixed in loading dye, were run in 1% agarose gel containing fluorescent dye of ethidium bromide to produce visible DNA bands.
Outcomes:
Neither smear nor DNA laddering band were detected upon the agarose gel.
Conclusions:
According to the present protocol, the UVB ray can not induce apoptosis feature in colonies of Bacillus anthracis. It seems that the recovery and protection systems in Bacillus anthracis can resist against eventual UVB disorders.
