Complete genome sequence of human adenovirus prototype 17.

Area of Science:

• Virology
• Genomics
• Bioinformatics

Background:

• Human adenovirus type 17 (HAdV-D17) was among the first HAdVs sequenced and served as a reference for comparative genomics of species D adenoviruses.
• The initial genome data for HAdV-D17, deposited in 1999, were of suboptimal quality, potentially impacting subsequent research.

Purpose of the Study:

• To re-sequence and correct the genome of the HAdV-D17 stock virus.
• To provide high-quality genomic data for accurate phylogenomic analyses and comparative studies of HAdV species D.

Main Methods:

• Redetermination of the HAdV-D17 genome sequence from the original stock virus.
• Comparative analysis of the newly determined genome against the previously published sequence.
• Annotation of coding sequences in the corrected genome.

Main Results:

• The corrected HAdV-D17 genome is 35,139 bp, differing from the original 35,100 bp.
• 160 mismatches were identified between the original and corrected genome sequences.
• The annotation revealed 39 coding sequences, a significant increase from the previously reported 8.

Conclusions:

• The re-sequencing provides a high-quality, accurate genome for HAdV-D17.
• The corrected genome and updated annotation are crucial for robust phylogenomic studies and understanding HAdV diversity.
• This improved reference genome facilitates more reliable comparative genomics of emerging HAdV pathogens.