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Updated: May 28, 2026

Single Oocyte Bisulfite Mutagenesis
Published on: June 27, 2012
Effect of postovulatory oocyte aging on DNA methylation imprinting acquisition in offspring oocytes
Xing-Wei Liang1, Zhao-Jia Ge, Lei Guo
1State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, People's Republic of China.
Objective:
To investigate whether postovulatory aging of oocytes in the mother affects DNA methylation acquisition of imprinted genes in oocytes from the offspring.
Design:
Randomized research experimental study.
Setting:
Academic basic research laboratory.
Animal(S):
Mice.
Intervention(S):
Fresh oocytes and aged oocytes from mothers were artificially inseminated, and oocytes were collected from the resultant offspring.
Main Outcome Measure(S):
Methylation status was evaluated at differentially methylated regions (DMRs) in oocytes of maternally imprinted genes Peg3, Snrpn, and Peg1 and paternally imprinted gene H19.
Result(S):
Our results showed that methylation patterns at DMRs of Peg3, Snrpn, Peg1, and H19 in oocytes from aged-oocyte offspring were mainly normal, with only a small number of oocytes showing aberrant methylation in the DMR of Peg3.
Conclusion(S):
Postovulatory oocyte aging causes a decline in reproductive outcomes but does not evidently lead to defects in DNA methylation imprinting acquisition in the oocytes from viable offspring.
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