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[The biological characteristics of micromycetes isolated from fish roe]
Abstract:
The fungi Aspergillus fumigatus Fres., and Fusarium sporotrichiella Bilai were studied for their effect on certain biochemical indices of two-year carp and spawn of grass carp under laboratory conditions. The intraperitoneal introduction of A. fumigatus in a dose of 5 and 20 min conidia per individual to two-year carp decreased significantly the protein level in blood serum, the decrease being more considerable with the introduction of the smaller dose of fungal conidia. Joint incubation of the grass carp spawn and F. sporotrichiella induced changes in the protein level, amylolytic and phosphate (acid phosphatase) activity in spawn. In that case the time of the fungus action on grass carp spawn was a decisive factor. Activity of certain hydrolytic enzymes in mycelium and conidia of A. fumigatus and F. sporotrichiella was determined. The amylolytic activity was not revealed in the checked samples. The proteolytic activity was established in all samples of fungi and culture liquid, the highest level being observed in mycelia and conidia of A. fumigatus. The alkaline and acid phosphatase activity was found in F. sporotrichiella: the acid phosphatase activity was higher in mycelium, the acid phosphatase one in the fungus conidia. The problem on the A. fumigatus ability to produce extracellular enzymes is under discussion.
Insights
Exposure to Aspergillus fumigatus and Fusarium sporotrichiella fungi significantly altered biochemical markers in carp and grass carp spawn. Fungal infections impacted protein levels and enzyme activity, with dose and exposure time being critical factors.
Area of Science:
- Aquatic toxicology
- Mycology
- Fish pathology
Background:
- Fungal infections pose a threat to aquaculture.
- Understanding the biochemical impact of fungi on fish is crucial for disease management.
Purpose of the Study:
- To investigate the effects of Aspergillus fumigatus and Fusarium sporotrichiella on biochemical indices in carp and grass carp.
- To determine the enzymatic activity of these fungi in vitro.
Main Methods:
- Laboratory exposure of two-year carp to A. fumigatus conidia via intraperitoneal injection.
- Incubation of grass carp spawn with F. sporotrichiella.
- Analysis of blood serum protein levels.
- Assay of amylolytic and phosphatase activity in spawn and fungal samples.
Main Results:
- A. fumigatus significantly decreased serum protein in carp, with a greater effect at a lower dose.
- F. sporotrichiella altered protein levels and enzyme activity in grass carp spawn, influenced by exposure duration.
- Proteolytic activity was detected in both fungi, highest in A. fumigatus.
- Phosphatase activity was found in F. sporotrichiella, with variations between mycelium and conidia.
Conclusions:
- Fungal pathogens A. fumigatus and F. sporotrichiella induce significant biochemical changes in fish.
- Enzyme production by these fungi contributes to their pathogenicity.
- Further research is needed on A. fumigatus's extracellular enzyme production.