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Updated: May 28, 2026

Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
Modulation of hypoxia-inducible factors (HIF) from an integrative pharmacological perspective
Francisco Javier Rodríguez-Jiménez1, Victoria Moreno-Manzano
1Neuronal Regeneration Laboratory, Centro de Investigacion Principe Felipe, Avd Autopista del Saler 16, 46012, Valencia, Spain. frodriguez@cipf.es
Abstract:
Oxygen homeostasis determines the activity and expression of a multitude of cellular proteins and the interplay of pathways that affect crucial cellular processes for development, physiology, and pathophysiology. Hypoxia-inducible factors (HIFs) are transcription factors that respond to changes in available oxygen in the cellular environment and drives cellular adaptation to such conditions. Selective gene expression under hypoxic conditions is the result of an exquisite regulation of HIF, from the pre-transcriptional stage of the HIF gene to the final transcriptional activity of HIF protein. We provide a dissected analysis of HIF modulation with special focus on hypoxic conditions and HIF pharmacological interventions that can guide the application of any future HIF-mediated therapy.
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