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Related Concept Videos

CRISPR01:59

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
What is Genetic Engineering?00:49

What is Genetic Engineering?

Overview
Homologous Recombination02:31

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: May 28, 2026

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
08:20

A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

Published on: September 2, 2021

More options for gene editing.

Monya Baker

    Nature Methods
    |October 11, 2011
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed novel gene-editing systems for precise genomic modifications in the model organism Caenorhabditis elegans. This breakthrough enables detailed study of gene function in this valuable research tool.

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    Last Updated: May 28, 2026

    A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization
    08:20

    A New Toolkit for Evaluating Gene Functions using Conditional Cas9 Stabilization

    Published on: September 2, 2021

    Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
    09:51

    Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

    Published on: May 25, 2018

    CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation
    08:31

    CRISPR/Cas9 Ribonucleoprotein-mediated Precise Gene Editing by Tube Electroporation

    Published on: June 20, 2019

    Area of Science:

    • Genetics and Genomics
    • Molecular Biology
    • Developmental Biology

    Background:

    • Precise genetic engineering is crucial for understanding gene function.
    • Gene-editing tools offer powerful applications in biological research.
    • Caenorhabditis elegans is a widely used model organism in biological studies.

    Discussion:

    • This study introduces the first systems for targeted genomic modifications in Caenorhabditis elegans.
    • The research was conducted in collaboration with Sangamo BioSciences.
    • The work was led by Howard Hughes Medical Institute investigator Barbara Meyer at the University of California, Berkeley.

    Key Insights:

    • Successful implementation of targeted genomic modification systems in C. elegans.
    • Establishes a foundation for advanced genetic studies in this model organism.
    • Demonstrates the utility of gene-editing tools for functional genomics.

    Outlook:

    • Potential for accelerated discovery of gene functions in C. elegans.
    • Opens new avenues for genetic manipulation in other model organisms.
    • Facilitates deeper understanding of developmental processes and disease mechanisms.