Reference intervals for saliva analytes collected by a standardized method in a physically active population.

Area of Science:

• Biochemistry
• Sports Medicine
• Clinical Chemistry

Background:

• Saliva analysis offers a non-invasive method for assessing various biomarkers.
• Establishing reliable reference intervals is crucial for interpreting saliva test results.
• Physically active populations may exhibit unique physiological responses impacting biomarker levels.

Purpose of the Study:

• To evaluate a liquid-based saliva collection system for TAS, UA, TP, and SAA.
• To investigate correlations between serum and saliva analytes in physically active males.
• To establish reference intervals for these salivary biomarkers.

Main Methods:

• 115 physically active males (18-20 years) provided saliva samples using a standardized collection system.
• Saliva was collected immediately prior to blood draws.
• Biochemical analyses were performed using an Autolab Boehringer analyzer.

Main Results:

• A significant correlation was observed between serum and saliva uric acid (UA) and total protein (TP) concentrations.
• Reference intervals for TP and salivary alpha-amylase (SAA) activity were lower in the morning compared to the afternoon.
• Reference intervals for UA and total antioxidant status (TAS) did not show significant variation based on collection time.

Conclusions:

• The validated saliva collection system provides a reliable method for assessing key biomarkers.
• Established reference intervals enhance the diagnostic utility of saliva analysis.
• Saliva biomarker analysis aids in detecting physiological or pathological states.