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Updated: May 28, 2026

16:16
Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors
Published on: September 13, 2013
Reengineering of a fluorescent zinc sensor protein yields the first genetically encoded cadmium probe
Jan L Vinkenborg1, Sander M J van Duijnhoven, Maarten Merkx
1Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, The Netherlands.
Abstract:
Introduction of a (Cys)(4) metal binding site at the dimerization interface of two fluorescent protein domains yields a chelating FRET sensor protein that shows a 2500-fold selectivity for Cd(2+) over Zn(2+) by taking advantage of their different ionic radii.
